Insider tips to optimize your GPCR signaling studies

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Drawing on dozens of data sets, this technical note gives you critical insights into the development, optimization, and validation of ERK phosphorylation assays. 

Get the experts’ opinion on:

  • Cell density 
  • Effect of serum starvation on signal
  • Stimulation time
  • Best practices in compound dispensing
  • Cell lysis and phosphorylation detection