Insider tips to optimize your GPCR signaling studies


Drawing on dozens of data sets, this technical note gives you critical insights into the development, optimization, and validation of ERK phosphorylation assays. 

Get the experts’ opinion on:

  • Cell density 
  • Effect of serum starvation on signal
  • Stimulation time
  • Best practices in compound dispensing
  • Cell lysis and phosphorylation detection