In this application note, Biotek’s Cytation™ 5 Cell Imaging Multi-Mode Reader and BioSpa were used to follow the expression of α-SMA in LX-2 cells in response to TGF-β stimulation. Phenotypic changes were captured using the instruments’ immunofluorescent and live imaging capabilities, and the quantitative expression of α-SMA was confirmed using the HTRF α-SMA kit.
This application note provides: