Technical note

Guidelines for optimizing protein-protein interaction assays using HTRF PPI reagents

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Technical note: HTRF protein-protein interaction assays

Every interaction is within range!

This Technical Note wants to help you develop the PPI assay of your dreams using HTRF®. As well as an introduction to the technology, it features the essential guidelines, with detailed instructions and microplate matrices, to develop, analyze, optimize and finally perform the assays you wish for.


  • Introduction to HTRF® assays and tips on experimental conditions
  • Step by step instructions and microplate matrices for each stage in development and optimization
  • Examples for proper data analysis

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